Co-Inoculation of Bacillus spp. for Growth Promotion and Iron Fortification in Sorghum

Seven Bacillus spp. isolated from the marine water and rhizosphere of medicinal plant Coscinium fenestratum were studied to produce growth promotion (PGP) traits invitro. Among seven isolates, MMRH22 RHPR20 produced copious amounts PGP traits. Based on 16S rRNA sequence, two potent bacterial MMRH22, identified as mojavensis cereus, respectively. A compatibility test between isolates revealed they are compatible can be used a consortium. Both evaluated for biofortification sorghum under greenhouse conditions. Treatments included application RHPR20, their consortium (RHPR20 + MMRH22), an uninoculated control. Inoculation with cultures resulted in significant increase height; number leaves; leaf area; root, shoot, weight; yield at 30 60 days after sowing (DAS). The scanning electron micrograph roots extensive colonization plants treated compared grains obtained final harvest analyzed nutrient content by ICP–OES. was varied found enhance iron up 97%. This study that treatments microbial consortia growth, yield, content, which could combat deficiencies humans.